Isolation, Cloning and Characterization of Oil Degrading Genes from Microbes and their Bioinformatics Analysis

Abstract

The isolation of new oil-degrading microbes from water or soil and the evaluation of their capacity to degrade oil were both very essential steps in the process of cleaning up oil-polluted habitats and extracting crude oil. For the quick and effective cloning of enzymatic genes of isolated oil degrading microbes, cloning was a relatively new method. Although the cloning method often relies on partial amino acid sequence in order to generate adequate DNA primers, the cloning process is tied to availability to accurate and sensitive enzyme tests. This method was quick and may potentially be used to characterize extracellular enzymatic genes. In this article, we also detailed the cloning procedures that are employed in the investigation and characterization of the enzymatic genes expression in microbes. In recent years, because to the revolution of microbial families, high-throughput sequence technologies have been created. These technologies, together with their analysis using appropriate bioinformatics methodologies, have played a vital role in the exploration of microbial genes. In conclusion, we covered the mechanisms of oil degradation from microbes, the isolation of oil degrading microbes, and the isolation of several oil degrading genes. categorized them using two distinct ways as follows: traditional physiochemical and 16S rRNA gene sequence analysis, as well as cloning and study of the expression of genes in microbes using bioinformatics.