PROTEOMIC ANALYSIS OF OCT4 PROTEIN, A MASTER REGULATOR OF REPROGRAMMING FACTOR OF INDUCED PLUROPOTENT CELLS (IPSCS)

Abstract

OCT4 is a component of a network of transcription factors, including Sox2, Klf4, and c-myc selected by Takahashi and Yamanaka to induce somatic cells into pluripotent stem cells (iPSCs). Several questions regarding reprogramming factors of stem cells are remaining unanswerable due to limited experimental availability and ehilical issues. Proteomic analysis of OCT 4 is still remaining unpredicted as protein structure is not available in PDB. Three dimensional structure of OCT4 has been modeled using homology modeling approach through MODELLER program. The modeled structure uploaded to PDBsum and obtained a set of password protected analysis for OCT4.  This paper shows annotated plots of each protein chain’s secondary structure, detailed structural analyses generated by the PROMOTIF program, protein?ligand interactions, PROCHECK analyses of structural quality, and many others. Stem cell research is used in treatment of a number of diseases including genetic disorders.