Screening and Identification of Asperginase and Glutaminase Producing Halophilic Bacteria From Natural Saline Habitats

Abstract

Halophiles are excellent sources of enzymes that are not only salt stable but also can withstand and carryout reaction efficiently under extreme conditions. Screening of halophiles from 5 different saline habitats led to the isolation of 34 bacterial isolates which showed glutaminase and asparaginase activity. Based on primary and secondary screening out of the 34 isolates, the 2 most potent strains were selected for further studies. Those were identified based on 16S rDNA sequencing. These isolates were also screened for various industrially important enzymes such as protease, amylase, phosphatase and cellulase. The present study is also focused on growth study, substrate optimization, and enzyme activity of 2 antineoplastic enzymes such as asparaginase and glutaminase at different time intervals.