Screening and Evaluation of Melanogenic Enzyme Tyrosinase Producing Bacterium from Different Crop Soil

Abstract

The present study is focused on the isolation and screening of tyrosinase enzyme produced by bacterial isolates and its application in the production of L-DOPA. 25 bacterial isolates have been screened which were collected from different crop soils. Based on primary screening, out of the 25 isolates the more potential tyrosinase producer was selected and identified by using 16S rDNA sequencing. The work also dealt with growth study, enzyme activity, L-DOPA production, extraction of melanin pigment, and chemical analysis. The pigment showed high absorbance in the UV region and decreased when shifted towards the visible region. The melanin pigment was further characterized by FT-IR Spectroscopy.